Enter the room blue with very low run speed (e.g. with a 4-tap). Use a precisely timed jump to bonk the large stalagtite in the ceiling and fall through both pirates, killing them with the blue speed. Enter the acid while aiming down to shrink Samus' hitbox. This strat works with all combinations of movement items and suits.

Entrance condition:

{
  "comeInGettingBlueSpeed": {
    "length": 9,
    "openEnd": 1,
    "gentleDownTiles": 4
  }
}

Requires:

{
  "notable": "Reverse Thread the Needle"
}
{
  "obstaclesNotCleared": [
    "A"
  ]
}
"canInsaneJump"
"canSuitlessLavaDive"
"canSlowShortCharge"
{
  "heatFrames": 330
}
{
  "acidFrames": 210
}